December 19, 1998
From: Roberto Moresco
Larval stage of Hypselodoris tricolor in laboratory is about 25 days, and the larvae continue eating Tetraselmis and Monocrisis. Hatching occurs after about 10 days. Intracapsular moving starts after after abaut 8 days.
We have some problems with protozoa even if we use sterile water (U.V.), because we take egg mass by pseudo natural system into which we leave the sponge Cacospongia (food of Hypselodoris).
We culture larvas in Capsule petri 50ml into which we put U.F.M.W. sponge as inducer, we don't use substratum because we are worried about problems with water quality.
Moresco@freeweb.amicus.itMoresco, R., 1998 (Dec 19) Re: Larvae of Hypselodoris tricolor. [Message in] Sea Slug Forum. Australian Museum, Sydney. Available from http://www.seaslugforum.net/find/407
Looks like you are having some success. Have you tried feeding the settled larvae on small pieces of Cacospongia. Some years ago, Geoff Avern, who was then a postgraduate student of mine, studied the growth of juvenile Noumea haliclona by carefully transferring the small juveniles on to new pieces of their food sponge every couple of days.
If you have photos of the larvae, the adults or the egg mass they would be interesting to put on the Sea Slug Forum. If you can't scan them yourself you could post me photos or slides and I will scan them here.
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